Transfer sponge pads for more reproducible Western blot transfers
• Resist compression - maintain proper pressure in transfer sandwiches without adding extra blotting papers
• Retain thickness - can be reused over multiple experiments
• Convenient - two sizes (8 x 11 cm and 9 x 15 cm) compatible with a variety of transfer apparatuses
• Standardized - uniform flat construction ensures reproducible and consistent transfers
Figure 1: The below diagram demonstrates the role of sponge pads in the transfer sandwich set-up, outside the blotting papers and just inside the transfer cassette. Sponge pads are important for making sure equal pressure is applied for even transfer from gel to membrane.