HRP-conjugated secondary antibodies for chemiluminescent immunodetection
• Performance – excellent signal in immunoblotting and ELISA applications
• Convenience – each antibody is demonstrated to provide optimal results with Advansta's protein detection systems
• Purity – each antibody is affinity purified to maximize specificity
Figure 1: Advansta secondary antibodies demonstrate increased sensitivity and specificity. HeLa, rat and mouse tissue lysates were separated by SDS-PAGE and transferred to a PVDF membrane. The blots were blocked with AdvanBlock™-Chemi before incubation with anti-Vinculin, anti-VDAC1 or anti-β Tubulin. Signal was detected with WesternBright® ECL. In addition to increased specificity, the blots probed with Advansta secondary antibodies demonstrate increased sensitivity compared to a leading competitor.