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SPHERO™ Rainbow Kalibrasyon Boncukları Geri

  • The Rainbow calibration particles, 8 peaks are designed for routine calibration of flow cytometers. Rainbow calibration particles, 8 peaks are commonly used in many laboratories for Quality Control and long-term performance of the analyser.

    Rainbow calibration particles, 8 peaks contain several similar sized particles that are dyed in eight different fluorescent intensities.  Each particle contains a mixture of fluorophores that are excited at any wavelength from 365 to 650 nm. The same set of particles enables the calibration of all channels in the flow cytometer. The Rainbow Particles have emission spectra compatible with many common fluorochromes used for immunofluorescent staining.

    Rainbow calibration particles, 8 peaks (Rainbow calibration, 8 peaks Cat. No. RCP-30-5A) are used in EuroFlow™ standardization guidelines throughout the study of initial PMT characterization, to set target MFI values and daily performance tracking of the flow cytometers.

    • EuroFlow™ reference target values (7th peak) for FACSCanto™ II

            Batch EAB01, EAC01, EAD05, EAE01, EAF01, EAG01 (check values)

     

    Lower MFI (-15%)

    Rainbow 7-peak (DiVa)

    Upper MFI (+15%)

    Pacific Blue™

    100.452

    118.178

    135.905

    Pacific Orange

    93.871

    110.436

    127.002

    FITC

    28.752

    33.826

    38.900

    PE

    32.381

    38.095

    43.809

    PerCP

    66.846

    78.642

    90.438

    PC7

    8.316

    9.783

    11.250

    APC

    158.639

    186.634

    214.629

    APC-H7

    64.194

    75.522

    86.850

    FACSCanto™ is a trademark of Becton, Dickinson and Company. Pacific Blue™ and Pacific Orange™ are trademarks of Life Technologies Corporation. Cy™ is a trademark of GE Healthcare. 
    EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO).

    • EuroFlow™ reference target values (7th peak) for Navios™ or Gallios™ 

            Batch EAB01, EAC01, EAD05, EAE01, EAF01; EAG01 (check values)

     

    Lower MFI (-15%)

    Rainbow 7-peak (Navios)

    Upper MFI (+15%)

    FL9 – Pacific Blue™

    379,874

    446,911

    513,948

    FL10 – OC515

    278,720

    327,906

    377,092

    FL1 - FITC

    117,719

    138,493

    159,267

    FL2 - PE

    101,813

    119,780

    137,747

    FL4 – PerCPCy™5.5

    253,075

    297,735

    342,395

    FL5 – PECy™7

    22,648

    26,645

    30,642

    FL6 - APC

    637,655

    750,182

    862,709

    AFL8 – APC-C750

    162,326

    190,972

    219,618

    Navios™ and Gallios™ are trademarks of Beckman Coulter Inc. Pacific Blue™ is a trademark of Life Technologies. Cy™ is a trademark of GE Healthcare. 
    EuroFlow™ is a trademark of European Scientific foundation for Laboratory Hemato Oncology (ESLHO). 

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