The best choice for routine, film-imaged chemiluminescent Western blots
• Stronger signal than Amersham™ ECL or Pierce™ ECL
• Optimized – for x-ray film imaging
• Sensitive – detect low pg protein amounts
• Lasting signal – up to 6 hrs
• Save money – use up to 10x less antibody
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution
WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. WesternBright ECL is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures without substantial signal decay.
WesternBright ECL is specially formulated to produce a strong signal for sensitive film imaging. WesternBright ECL substrate produces a strong, long-lasting signal after reaction with HRP. Duplicate slot blots containing serial dilutions of transferrin protein were detected using WesternBright ECL or Amersham™ ECL™ (GE Healthcare). Both blots were simultaneously exposed to the same sheet of film for 15 seconds. WesternBright ECL is several times more sensitive than Amersham ECL.
The higher sensitivity of WesternBright ECL is especially important when detecting low abundance proteins. Duplicate Western blots with serial dilutions of HeLa cell lysate were probed for ERK1 and detected using either WesternBright ECL or Amersham ECL HRP substrates. Each blot was exposed to film for 1 minute.
Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody diutions shown, and detected with either Westernbright ECL or Pierce ECL (Thermo Scientific) according to the manufacturer’s instructions. The blots were imaged simultaneously on the same piece of film. WesternBright ECL produced the same sensitivity with ten times less primary and five times less secondary antibody.
WesternBright ECL produces a long-lasting signal. A blot detected with WesternBright ECL can be imaged six hours after substrate incubation. There is no need to rush to the darkroom to image a blot.
The greater stability of WesternBright ECL allows more signal to be accumulated during long exposures, increasing the ability to detect very low abundance proteins. Duplicate Western blots were probed for ATP1A1 protein and detected using WesternBright ECL or Amersham ECL. In a 10 minute exposure, only 2 bands can be detected with either substrate. With a 60 minute exposure, a band is visible in the 1.25 μg lane of the WesternBright ECL blot, while signal decay results in little difference between a 10 min or 60 minute exposure of the Amersham ECL blot
WesternBright ECL produces a strong signal resulting in greater sensitivity with much shorter exposure times. Duplicate blots containing serial dilutions of transferrin protein were detected using WesternBright ECL or Amersham ECL according to the manufacturers' instructions, and the blots were exposed to the same piece of film. More bands can be detected with a 30 second exposure of the WesternBright ECL blot than with a 5 minute exposure of the Amersham ECL blot.